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Gene amplification PCR amplifications were performed
2019-11-06
Gene amplification. PCR amplifications were performed using genomic DNA from the different organisms studied as template and two degenerate oligonucleotides (PP1 and PP2) as primersPP1 corresponded to the sense codons of the amino BGP-15 motif AGGI(A/S)EM, and PP2 to the antisense sequence of the m
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br Materials and methods br
2019-11-06
Materials and methods Results Discussion Our previous investigation demonstrated that some genes in the latex that were involved in rubber biosynthesis were regulated by exogenous JA, and Hbvp1 is one of the JA-responsive genes in the latex of rubber trees [23]. In this study, the Hbvp1 enc
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In conclusion using human liver microsomes supersomes
2019-11-06
In conclusion, using human liver microsomes, supersomes from baculovirus-transformed insect cells expressing different human CYP450 isoforms, and V79MZh3A4 cells, MB2 and MB4 were formed from TRB and MC from TRC. The amounts of MB2, MB4, and MC formed were related to the 6β-testosterone hydroxylase
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Although the reasons why viral RNA does
2019-11-06
Although the reasons why viral RNA does not bind to and activate PKR in WNV-infected cells are not known, some of the known characteristics of flavivirus infections provide possible clues. The viral Caspase-3/7 Inhibitor protein forms dimers that associate with ER membranes and viral RNA (Lindenbac
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br Results and discussion br Conclusion Recent studies showe
2019-11-06
Results and discussion Conclusion Recent studies showed that EGFR signaling is involved in inflammatory response in several inflammatory conditions. The normal inflammatory macrophage Acetaminophen show EGFR-dependent production of inflammatory mediators. Nevertheless, the development of EGFR
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kanamycin sulfate Previously we showed that MS
2019-11-06
Previously, we showed that MS-10, a combined extract of Cirsium japonicum var. Maackii (Maxim.) Matsum. (Korean milk thistle) and Thymus vulgaris L., increases the efficiency of estrogen in the estrogen-deficient state by activating the estrogen receptor (ER). MS-10 increased bone mineral density (B
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Advances in the total chemical
2019-11-06
Advances in the total chemical synthesis of ubiquitin have enabled the efficient synthesis of new and improved ubiquitin-based reagents. Using an optimized linear synthesis, ubiquitin can now be easily obtained in high yield and purity [35]. Using this synthetic methodology, ubiquitin can be functio
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salinomycin The question remained as to why hCrm was
2019-11-06
The question remained as to why hCrm1 was functional and mCrm1 was not in terms of Rev and HIV mRNA nuclear export, and previously we proposed three possibilities (Elinav et al., 2012). One was that human salinomycin had a positively acting factor that somehow stabilized the hCrm1 and Rev-RRE comple
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Converging evidence suggests that reduced glutamate transmis
2019-11-06
Converging evidence suggests that reduced glutamate transmission in the LS is linked to increased anxiety-like behaviors (Radulovic et al., 1999, Henry et al., 2006, Bakshi et al., 2002, Liu et al., 2004). Therefore, attenuated CRF1 receptor-mediated excitatory transmission in the LS following ASV-t
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Alternatively procedural factors may have contributed to the
2019-11-06
Alternatively, procedural factors may have contributed to the conflicting results. For instance, in order to have a more circumscribed area of drug diffusion, a volume of 0.05μl was injected in experiment 1b, which is four times lower than the one used in the mentioned plus-maze investigation. Besid
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RO4929097 br Acknowledgements We thank Jeus Perez Clausell
2019-11-06
Acknowledgements We thank Jeus Perez-Clausell from the Department of Cell Biology, School of Biology, University of Barcelona, Felipe Casanueva\'s group from the Department of Molecular Endocrinology and Carlos Diéguez\'s group from the Department of Physiology, School of Medicine, University of
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Iris domestica L Goldblatt Mabb
2019-11-06
Iris domestica (L.) Goldblatt & Mabb. (syn. Belamcanda chinensis L.) DC. (Iridaceae) occurs naturally in South and East Asia. Despite the fact that former monotypic genus Belamcanda has been included in the extended genus Iris, this classification has been questioned (Wilson, 2011; Mavrodiev et al.,
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Despite many studies having demonstrated the importance of
2019-11-06
Despite many studies having demonstrated the importance of connexin43 (Cx43), the most abundant connexin in bone cells, for bone development and turnover during the last decade [27], little is currently known about GJIC and Cx43 in primary bone tumors. In this report, we analyzed the specific role o
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The synthetic routes to the substrates based on the
2019-11-06
The synthetic routes to the substrates based on the 2,3-dihydroxynaphthalene and 6,7-dibromo-2,3-dihydroxynaphthalene cores are depicted in Scheme 3. A Michael-type glycosylation of 2,3-dihydroxynaphthalene 16a gave the acetylated sugar 17a which was deprotected giving the required β-glucosidase sub
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br Material and methods br
2019-11-06
Material and methods Results Discussion Cysticerci located in host tissues are exposed to several effector mechanisms of the immune system (Flisser et al., 1986; Sciutto et al., 2000; Amit et al., 2011; Singh et al., 2013). In addition, the extensive uptake of host proteins by cysticerci ha
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